human iga2 antibody  (NSJ Bioreagents)

Journal: Scientific Reports

Article Title: SARS-CoV-2-specific B cell responses in non-draining lymph nodes and antibody functionalities in immunized end-stage renal disease patients

doi: 10.1038/s41598-025-27815-y

Figure Lengend Snippet: The B cell isotypes in the LN and PB compartment, the horizontal black lines indicate the average percentage in both compartments ( a ) IgM in switched B cells, lower in LNs than in PB at 8% (IQR: 5% − 11%) or 13% (IQR: 11% − 26%) ( p = 0.0001), respectively; ( b ) IgM in S-binding B cells, no difference between the LNs and PB at 29% (IQR: 16% − 51%) or 15% (IQR 6% − 33%) ( p = 0.058), respectively; ( c ) IgA in switched B cells, no difference between LNs and PB at 36% (IQR 27% − 43%) or 37% (IQR 32% − 43%) ( p = 0.207), respectively; ( d ) IgA in S-binding B cells, in LNs higher than in PB at 22% (IQR 11% − 32%) or 8% (IQR 5% − 15%) ( p = 0.0002), respectively; ( e ) IgG in switched B cells, in LNs higher than in PB at 49% (IQR 42% − 53%) or 42% (IQR 32% − 48%) ( p = 0.0110), respectively; ( f ) IgG in S-binding B cells, in LNs lower than in PB at 53% (IQR: 44% − 67%) or 73% (IQR 61% − 80%) ( p = 0.0001), respectively. PRM (primary), two vaccinations; BST (booster three), three vaccinations; BSF (booster four), four vaccinations; INFX (infection), prior infection; HYB (hybrid), immunity in which the infection was prior to the last of two vaccinations.

Article Snippet: The following surface mAbs were used: CD19 BV785, CD20 APC-Fire750, CD27 BB700, IgD PE-CF594, CD24 BV650, CD38 BUV563, IgM BUV395, IgG BUV496 (all from BD Biosciences), CXCR3 R718 (eBioscience), IgG1 PE, IgG2 PE, IgG2 FITC, IgG3 FITC (all from SouthernBiotech), IgA PE-Vio770, IgA2 FITC (both from Miltenyi Biotec), and IgA1 PE (Abcam).

Techniques: Binding Assay, Infection

Journal: Journal of medical microbiology

Article Title: Comprehensive analysis of human coronavirus antibody responses in ICU and non-ICU COVID-19 patients reveals IgG3 against SARS-CoV-2 spike protein as a key biomarker of disease severity.

doi: 10.1099/jmm.0.002012

Figure Lengend Snippet: Fig. 2. Total IgG, IgM and IgA antibody responses against SARS-CoV-2 antigens in ICU and non-ICU patients. (a) Frequency of positive Ig response in ICU and non-ICU patients. IgM, IgG and IgA RIs to SARS-CoV-2 antigens for (b) Envelope, (c) Nucleo, (d) Spike protein trimer and its fragments, (e) S1 and (f) RBD. A horizontal straight line represents the means, and the bars represent the sem. Unpaired two-tailed t-tests corrected for multiple comparisons by the Holm–Šidák correction were used to compare the ICU and non-ICU patients. (g) Heatmap analysis of the average of total IgG, IgM and IgA antibody responses against HCoVs S1 subunits. Yellow, green and blue indicate high, medium and low positivity index average values according to the scale of log2 of RIs. The significance symbols are plotted on the highest RI for each reactivity. Significance levels *: 0.01≤P≤ 0.05, **: 0.001≤P<0.01 and ***: P<0.001.

Article Snippet: The microspheres in the other plate were incubated with 50 μl of assay buffer containing 1 μg ml−1 of PE- labelled mouse monoclonal antibody B3506B4 anti- IgA1 Fc (SouthernBiotech, catalogue # 9130–30) and 1 μg ml−1 of APC- labelled mouse monoclonal antibody A96042 anti- IgA2 Fc (SouthernBiotech, catalogue # 9140–31).

Techniques: Two Tailed Test

Journal: Journal of medical microbiology

Article Title: Comprehensive analysis of human coronavirus antibody responses in ICU and non-ICU COVID-19 patients reveals IgG3 against SARS-CoV-2 spike protein as a key biomarker of disease severity.

doi: 10.1099/jmm.0.002012

Figure Lengend Snippet: Fig. 3. Subclass of IgG and IgA antibody responses against SARS-CoV-2 antigens in ICU and non-ICU patients. (a) Frequency of positive Ig response to SARS-CoV-2 antigens. RIs to SARS-CoV-2 antigens for (b) Envelope, (c) Nucleo, (d) Spike protein trimer and its fragments, (e) S1 and (f) RBD. A horizontal straight line represents means, and the bars represent the sem. Unpaired two-tailed t-tests corrected for multiple comparisons by the Holm–Šidák correction were used to compare the ICU and non-ICU patients. (g) Heatmap analysis of the average of total IgG, IgM and IgA antibody responses against HCoVs S1 subunits. Yellow, green and blue indicate high, medium and low positivity index average values according to the scale of log2 of RIs. Significance levels *: 0.01≤P≤ 0.05, **: 0.001≤P<0.01 and ***: P<0.001.

Article Snippet: The microspheres in the other plate were incubated with 50 μl of assay buffer containing 1 μg ml−1 of PE- labelled mouse monoclonal antibody B3506B4 anti- IgA1 Fc (SouthernBiotech, catalogue # 9130–30) and 1 μg ml−1 of APC- labelled mouse monoclonal antibody A96042 anti- IgA2 Fc (SouthernBiotech, catalogue # 9140–31).

Techniques: Two Tailed Test

Journal: Journal of medical microbiology

Article Title: Comprehensive analysis of human coronavirus antibody responses in ICU and non-ICU COVID-19 patients reveals IgG3 against SARS-CoV-2 spike protein as a key biomarker of disease severity.

doi: 10.1099/jmm.0.002012

Figure Lengend Snippet: Fig. 4. Total IgG, IgM and IgA antibody responses against HCoVs S1 proteins in ICU and non-ICU patients. (a) Frequency of positive Ig response. IgM, IgG and IgA RIs to HCoVs S1 proteins for (b) SARS-CoV-2, (c) SARS, (d) MERS, (e) 229E, (f) NL63, (g) HUK1 and (h) OC43. A horizontal straight line represents the mean, and the bars represent the sem. Unpaired two-tailed t-tests corrected for multiple comparisons by the Holm–Šidák correction were used to compare the ICU and non-ICU patients. (i) Heatmap analysis of the average of total IgG, IgM and IgA antibody responses against HCoVs S1 subunits. Yellow, green and blue indicate high, medium and low positivity index average values according to the scale of log2 of RIs. Significance levels *: 0.01≤P≤0.05, **: 0.001≤P<0.01 and ***: P<0.000.1.

Article Snippet: The microspheres in the other plate were incubated with 50 μl of assay buffer containing 1 μg ml−1 of PE- labelled mouse monoclonal antibody B3506B4 anti- IgA1 Fc (SouthernBiotech, catalogue # 9130–30) and 1 μg ml−1 of APC- labelled mouse monoclonal antibody A96042 anti- IgA2 Fc (SouthernBiotech, catalogue # 9140–31).

Techniques: Two Tailed Test

Journal: Journal of medical microbiology

Article Title: Comprehensive analysis of human coronavirus antibody responses in ICU and non-ICU COVID-19 patients reveals IgG3 against SARS-CoV-2 spike protein as a key biomarker of disease severity.

doi: 10.1099/jmm.0.002012

Figure Lengend Snippet: Fig. 5. Subclass of IgG and IgA antibody responses against HCoVs S1 proteins in ICU and non-ICU patients. (a) Frequency of positive Ig subclass response to S1 proteins. RIs to HCoVs S1 proteins for (b) SARS-CoV-2, (c) SARS, (d) MERS, (e) 229E, (f) NL63, (g) HUK1 and (h) OC43. A horizontal straight line represents the mean, and the bars represent the sem. Unpaired two-tailed t-tests corrected for multiple comparisons by the Holm–Šidák correction were used to compare the ICU and non-ICU patients. (i) Heatmap analysis of the average of total IgG, IgM and IgA antibody responses against HCoVs S1 subunits. Yellow, green and blue indicate high, medium and low positivity index average values according to the scale of log2 of RIs. Significance levels *: 0.01≤P≤ 0.05, **: 0.001≤P<0.01 and ***: P<0.001.

Article Snippet: The microspheres in the other plate were incubated with 50 μl of assay buffer containing 1 μg ml−1 of PE- labelled mouse monoclonal antibody B3506B4 anti- IgA1 Fc (SouthernBiotech, catalogue # 9130–30) and 1 μg ml−1 of APC- labelled mouse monoclonal antibody A96042 anti- IgA2 Fc (SouthernBiotech, catalogue # 9140–31).

Techniques: Two Tailed Test